Abstract
Introduction: Chronic kidney disease (CKD) is considered a very serious worldwide health problem. Renalase is flavin adenine dinucleotidedependent amine oxidase. It is secreted from the kidney. Renalase release in blood to regulate cardiac function as systemic blood pressure. Relative decrease in renalase enzyme in patients with CKD can be a cause of occurrence of hypertension in CKD patients and an increased risk of cardiovascular complications in end stage renal disease (ESRD) patients.
Objectives: In this study, we tried to study the association of the two renalase gene polymorphisms rs2296545 and rs10887800 in ESRD patients and occurrence of hypertension in Egyptian prevalent hemodialysis (HD) patients.
Patients and Method: Rs2296545 polymorphism and rs10887800 polymorphism were genotyped in 337 subjects that divided into 2 groups: group I; 252 prevalent HD patients that subdivided into two subgroups: Subgroup Ia included 187 subjects with hypertension and subgroup Ib included 65 subjects without hypertension. Group II included 85 healthy control subjects. We used the technique of polymerase chain reaction (PCR), followed by cleavage with Eco81 I restriction endonuclease for rs2296545 polymorphism and Pst I restriction endonuclease for rs10887800 polymorphism.
Results: Comparison showed that rs2296545 CC genotype showed significant increase in HD patients when compared to healthy control and that rs2296545 CC genotype showed significant increase in HD patients with hypertension than those without hypertension. In addition, rs10887800 AA genotype showed significant increase in HD patients when compared to healthy control.
Conclusion: Renalase gene polymorphisms might have role in pathophysiology of ESRD especially in hypertensive patients.